Table 2.
Expression ratio of peptide transporter genes HaPTR4, HaPTR5, and HaPTR12 in hydroponically grown Hakea actites
| Tissue | Transporter | Values | No N | 15 μM N concentration |
150 μM N concentration |
||||
| BSA | Glycine | NH4NO3 | BSA | Glycine | NH4NO3 | ||||
| Cluster root | HaPTR4 | Expression (×10−5) | 6.67±4.28 | 4.77±4.05 | 11.75±7.49 | n/a | n/a | n/a | n/a |
| Comparative | 1 | 1.4 | 0.6 | n/a | n/a | n/a | n/a | ||
| HaPTR5 | Expression (×10−5) | 6.36±3.82 | 5.45±4.05 | 12.17±8.13 | n/a | n/a | n/a | n/a | |
| Comparative | 1 | 1.2 | 0.5 | n/a | n/a | n/a | n/a | ||
| HaPTR12 | Expression (x10-5) | 4.65±3.64 | 2.16±0.50 | 14.57±1.02 | n/a | n/a | n/a | n/a | |
| Comparative | 1 | 2.2 | 0.3 | n/a | n/a | n/a | n/a | ||
| Non-cluster root | HaPTR4 | Expression (×10−5) | 15.78±15.43 | 23.10±24.96 | 8.82±9.63 | 6.13±5.01 | 5.36±0.92 | 4.77±5.06 | 2.42±1.14 |
| Comparative | 1 | 0.7 | 1.8 | 2.6 | 2.9 | 3.3 | 6.5 | ||
| HaPTR5 | Expression (×10−5) | 10.03±9.29 | 14.33±15.33 | 4.00±4.02 | 4.44±4.71 | 2.74±0.78 | 0.98±0.15 | 1.28±0.59 | |
| Comparative | 1 | 0.7 | 2.5 | 2.3 | 3.7 | 10.2 | 7.8 | ||
| HaPTR12 | Expression (×10−5) | 7.14±7.28 | 7.26±7.47 | 4.15±5.21 | 2.23±1.99 | 1.58±0.38 | 3.75±5.83 | 0.65±0.27 | |
| Comparative | 1 | 1.0 | 1.7 | 3.2 | 4.5 | 1.9 | 11.0 | ||
| Leaves | HaPTR4 | Expression (×10−5) | 28.32±21.62 | 20.12±15.18 | 24.75±5.43 | 15.07±9.19 | 12.69±9.80 | 4.91±2.45 | 14.72±11.1 |
| Comparative | 1 | 1.4 | 1.1 | 1.9 | 2.2 | 5.8 | 1.9 | ||
| HaPTR5 | Expression (×10−5) | 4.23±4.09 | 1.39±0.95 | 1.66±0.17 | 1.29±0.59 | 0.63±0.42 | 1.03±0.99 | 1.12±0.75 | |
| Comparative | 1 | 3.0 | 2.5 | 3.3 | 6.7 | 4.1 | 3.8 | ||
| HaPTR12 | Expression (×10−5) | 11.03±8.79 | 7.40±5.54 | 11.79±4.53 | 5.11±3.81 | 6.46±5.39 | 2.84±2.16 | 5.75±4.82 | |
| Comparative | 1 | 1.5 | 0.9 | 2.2 | 1.7 | 3.9 | 1.9 | ||
Plants were cultivated without N for 3 months and then grown for 7 months with different N treatments. Data represent averages ±SDs of three independent replicates. Expression values were normalized by comparing them with 18S rRNA, and comparative values are the expression results of plants grown on different N compared with expression of plants grown without N supply. See Table 1 for biomass and number of cluster roots.
n/a, plants produced no or insufficient (15 μM NH4NO3) clusters.