Table 1.
Summary of ripening treatments for MdACO1as and control ‘Royal Gala’ apples
| Treatment no. | Genotype | Ethylene dosage (μl l−1) | Ethylene treatment duration | Ripening temperature (°C) | 600 nl l−1 MCP treatment |
| 1 | Control | – | – | 20 | No |
| 2 | – | – | 20 | Yes | |
| 3 | – | – | 4 | No | |
| 4 | ACO1as | – | – | 20 | No |
| 5 | – | – | 4 | No | |
| 6 | – | – | 20 | Yes | |
| 7 | 0.01 | 14 d | 20 | No | |
| 8 | 0.1 | 14 d | 20 | No | |
| 9 | 1 | 14 d | 20 | No | |
| 10 | 10 | 14 d | 20 | No | |
| 11 | 100 | 14 d | 20 | No | |
| 12 | 1000 | 14 d | 20 | No | |
| 13 | 100 | 30 min | 20 | Yes | |
| 14 | 100 | 4 h | 20 | Yes | |
| 15 | 100 | 1 d | 20 | Yes | |
| 16 | 100 | 7 d | 20 | Yes |
Ripening treatments included ethylene dosage, duration of ethylene treatment, 1-methylcyclopropene (MCP) treatment, and ripening temperature. Assessments for each treatment were performed 14 d after harvest.