Fig. 2.

Response of EguCBF1 genes to different environmental stimuli. Total RNA was extracted from leaf discs of E. gundal exposed for 2 h to cold (4 °C), NaCl (200 mM), heat (50 °C), drought (22 °C dessication) or exogenous ABA (100 μM). For evaluating the wounding effect, the leaf discs (distilled water, 22 °C) were compared to control whole leaves. Relative abundance of EguCBF1 transcripts was quantified in comparison to the 18S RNA transcript level using RT-qPCR with gene-specific primers (see Supplementary Table S2 at JXB online). The results correspond to the mean value of three assay replicates compared to the mean of the three control values. After normalization of the RNA steady-state level using 18S as an internal control, the EguCBF1 transcript levels of control tissues was used as a calibrator to determine the fold change of transcript abundance during treatment. A representative histogram with standard deviation from three replicates performed for each time point has been represented.