Fig. 3.

Time-course of EguCBF1 gene expression at 4 °C in the dark. Total RNA was extracted from a pool of leaves harvested before cold exposure (control), after 15 min, 30 min, 2 h, 5 h, and 24 h. (A) The relative EguCBF1 transcript abundance was determined as described in the legend of Fig. 2. (B) Copy number ng⁻¹ of cDNA of EguCBF1 genes was calculated using a standard curve (see the Materials and methods for details). For transcript copy number ng⁻¹ of cDNA values, closed circles, open triangles, open circles, closed triangles, and closed squares correspond to EguCBF1a, EguCBF1b, EguCBF1c, EguCBF1d genes, and the total amount of the EguCBF1 genes, respectively.