Fig. 5.

EguCBF1 transcript accumulation during an acclimation programme of plantlets in short-day conditions (SD). Total RNA was extracted from Eucalyptus leaves randomly harvested at different times during the cold programme: 30 min, 2 h after transfer to 12 °C (D1), and 30 min, 2 h, and 5 h after transfer to 4 °C (D5), and at the end of each day (except D6 and D8). The copy number ng⁻¹ of cDNA of EguCBF1 during the plantlet acclimation programme was quantified using RT-qPCR as described in the legend of Fig. 2 and in the Materials and methods. For transcript copy number ng⁻¹ of cDNA values, closed circles, open triangles, open circles, closed triangles, and closed squares correspond to EguCBF1a, EguCBF1b, EguCBF1c, EguCBF1d, and the total amount of the EguCBF1 genes, respectively.