Fig. 1.

(A) Sensitivity to cisplatin of single mutants atbrca2a-1 and atbrca2b-1, and the double mutant atbrca2a-1/atbrca2b-1. Imbibed seeds were plated on MS agar medium containing 0–50 μM cisplatin. The numbers of true leaves in wild type (Nossen) and mutant plants were counted 14 d after plating. Data represent the mean ±SE of 50 plants in each group from three experiments. (B, C) Sensitivity of AtBRCA2 mutants to γ-irradiation. (B) Imbibed seeds (4 d at 4 °C) were irradiated with increasing doses of ⁶⁰Co γ-rays. After γ-irradiation, the seeds were immediately plated on MS agar medium. The number of true leaves was counted 10 d after irradiation, and the average number of leaves was calculated according to Harlow et al. (1994). Data represent the mean ±SE of 34 plants in each group from three experiments. (C) Fourteen-day-old plantlets [upper panels: wild type (Nossen), lower panels: atbrca2a-1/atbrca2b-1 double mutant plants] after γ-irradiation (0, 300, 600 Gy).