Fig. 6.

Effects of PwTUA1 overexpression on plant pollen germination and pollen tube growth exposed to different concentrations of boron and Ca²⁺. (A) Comparison of tube growth of wild-type (WT) and transgenic plant pollen cultured in medium with 0.1% H₃BO₃ for 6 h. Only the pollen tubes of germinated pollen grains were measured. (B) Arabidopsis pollen was incubated in the germination medium with the indicated amounts of Ca²⁺. After 6 h of incubation, the percentage of germinating pollen grains was determined. To establish the pollen germination percentage, the percentage germination of pollen grains in at least 10 aliquots per treatment was determined and experiments were repeated three times. (C) Statistical analysis of pollen tube length after 6 h of incubation. Only the pollen tubes of germinated pollen grains were measured. Average lengths of >100 tubes from each line are shown. The experiments were repeated three times, and consistent results were obtained in all repeat experiments.