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. 2009 May 19;60(9):2737–2749. doi: 10.1093/jxb/erp143

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© 2009 The Author(s).

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

This paper is available online free of all access charges (see http://jxb.oxfordjournals.org/open_access.html for further details)

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Fig. 8. — Effects of PwTUA1 on the ultrastructure of pollen tubes in transgenic Arabidopsis. Pollen from wild-type and transgenic plants was cultured in vitro for 6 h. Bars=0.2 μm (A, B, E, F) or 0.5 μm (C, D). (A) Tip region of wild-type pollen tube showing organelles at the tip. (B) Tip region of a pollen tube expressing PwTUA1; MTs (arrowhead) appeared and the number of vacuoles and secretory vesicles increased. (C and D) Magnified images of boxed sections in A and B. (E) Pollen tube wall of wild-type Arabidopsis. (F) Pollen tube wall of transgenic Arabidopsis. For Arabidopsis expressing PwTUA1, analyses of two independent transgenic lines showed a similar ultrastructure of pollen tubes. Data from one transgenic line were used. CW, cell wall; Mi, mitochondria; D, dictyosome; SV, secretory vesicle; V, vacuole; L, lipid body.