Fig 2. Protein tyrosine phosphorylation and phosphorylation of LAT/NTAL in response to antigen and/or PGE₂.

A, B. BMMCs were sensitized overnight and treated with antigen (Ag), PGE₂, or Ag/PGE₂ concurrently for the indicated times, and protein samples were prepared as described in “Materials and Methods”. Following gel electrophoresis, the whole-cell extracts were immunoblotted with anti-phosphotyrosine (4G10-HRP) (A) or anti-phospho-LAT (Y191) antibodies (B). The blots are representative of three to five independent experiments. Protein loading of the samples was normalized by stripping and then probing for β-actin. C. The data were generated by the scanning the blots in B in three independent experiments, then normalizing to the response at 120 seconds obtained with Ag/PGE₂. The data in C are presented as means ± S.E. of (n=3) separate experiments.