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. Author manuscript; available in PMC: 2009 Oct 9.
Published in final edited form as: Nature. 2009 Mar 11;458(7239):725–731. doi: 10.1038/nature07782

Figure 4. Effects of modulation of PI3K signaling on the apoptotic response of tumours to DR.

Figure 4

a-d, Immunohistochemical analyses of FOXO1 (a, b) and cleaved caspase-3 (c, d) in tumours formed by DLD-WT and DLD-Mut cells and by the PTEN-inducible U87-MG cells in mice treated or non-treated with doxycycline (Dox). Graphs to right of images indicate percent of total cells that are positive for cleaved caspase-3. Data in c and d graphs are means ± s.e.m, measured in 9 images (1000 nuclei counted per image) from 3 different tumours per group. ** indicates P ≤ 0.01. All images were acquired at the same magnification and scale bar = 20 µm. Framed inserts in a and b are a 3.9-fold magnification of a representative area of the corresponding larger image. Arrows point to immunoreactivity for FOXO1 (a, b) or cleaved caspase-3 (c and d).