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. Author manuscript; available in PMC: 2010 Apr 3.
Published in final edited form as: J Proteome Res. 2009 Apr 3;8(4):1810–1817. doi: 10.1021/pr8004919

Figure 2.

Figure 2

Stability of rSET and modified porcine trypsin against autolysis. rSET and modified porcine trypsin were incubated in 50 mM ammonium bicarbonate at 37 °C for different duration (0, 6, 12, 24, 48, and 96 h) and subjected to SDS-PAGE analysis (A) and amidase activity assay, -●-: rSET, -○-: modified porcine trypsin (B). Protein bands were quantified by densitometry using DeCyder software (GE Health Care) and normalized to 0 time. The result of activity assay are means ± standard deviation from triplicate reactions.