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Effect of urea (A) and Gdn-HCl (B) on the amidase activity of rSET and modified porcine trypsin. rSET or modified porcine trypsin (170 nM) was incubated with 10 mM Bz-ArgpNA in 100 mM Tris-HCl pH 8.0 buffer in the presence of various amount of urea or Gdn-HCl at 25°C. -●-: rSET, -○-: modified porcine trypsin. The result of activity assay are means ± standard deviation from triplicate reactions.
