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Discontinuous adenine-luciferase assay format kinetic curve fits for RTA catalysis of stem-loop RNA A-10 (A) and A-14 2-dA (B) at pH 4.0. Kinetic curve fits for RTA catalysis of 80S rabbit (C) and 60S yeast (D) ribosome at pH 7.4 in 20 mM tris-HCl, 25 mM KCl, and 5 mM MgCl2.
