Table 1.

RTA kinetic parameters

Substrate	Km (µM)	kcat (min−1)	kcat / Km (M−1s−1)
A-10 d*	2.2 ± 0.3	4.7 ± 0.3	3.6 × 104
A14 2-dA d*	10.3 ± 1.6	1110 ± 50	1.8 × 106
60S yeast ribosome d	0.270 ± 0.08	460 ± 90	2.8 × 107
60S yeast ribosome c	0.240 ± 0.06	270 ± 40	1.9 × 107
80S rabbit ribosome d	0.150 ± 0.025	2310 ± 150	2.6 × 108
80S rabbit ribosome c	0.100 ± 0.005	820 ± 10	1.4 ×108

^dsubstrate assayed in discontinuous format

^csubstrate assayed in continuous format

^*substrate assayed at 37 °C