Figure 5.

Effects of Olfm1–MO injections on the eyes of 72 hpf embryos. A, B, Embryos were injected with 5 ng of control (A) or Olfm1–MO (B). Olfm1–MO-injected embryos showed a reduction of eye size and pigmentation compared with embryos injected with control MO. C, D, TUNEL staining of eye sections from embryos injected with 5 ng of control MO (C) or Olfm1–MO (D). E, F, In 3 days postfertilization embryos, Olfm1–MO (F) inhibited trkc1 expression in both RGC and inner plexiform layers compared with control MO (E). Scale bar, 0.05 mm. IPL, Inner plexiform layer; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; RPE, retinal pigmented epithelium; RGC, retinal ganglion cells.