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Olfm1 is required for axonal extensions. A, B, DiI-labeled optic nerve of 80 hpf control (A) and Olfm1–MO-injected embryos (B). A total of 1.25 ng of Olfm1–MO produced inhibition of axon growth to the optic tectum. C, D, Control (C) and Olfm1–MO-treated (D) 35 hpf embryos were stained with antibodies against acetylated tubulin. Note strong inhibition of neurons and their networks in Olfm1–MO-injected embryos. LLn, Lateral line nerve. Scale bar, 0.1 mm.
