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Changes in the expression patterns of early eye markers after olfm1 RNA or Olfm1–MO injection. Embryos were injected with 0.1 ng of zOlfm1 RNA (F), 5 ng of Olfm1–MO, and 0.1 ng of EGFP RNA (B, E) or a mixture of zOlfm1 RNA and Olfm1–MO (C) and were analyzed for rx3 (A–C), and six3 (D–F) mRNA expression at 13 hpf. Control embryos (A, D) were injected with 0.1 ng of EGFP RNA. Shown are dorsal views of the future heads. a, Anterior; p, posterior. Scale bar, 0.1 mm.
