Figure 2.
Long-lasting induction of ImGluR(V) by DHPG. A, Whole-cell patch-clamp recordings of responses of a CA3 pyramidal cell to DHPG. Hyperpolarizing voltage steps (inset) were applied every 30 s throughout the recording. B, Current responses to pulses in A are shown expanded in the insets. The net inward current elicited by DHPG (ImGluR(V)) after the pulses (segment between arrows in the voltage protocol in a) was obtained by subtracting the control response (*) from the corresponding traces. C, Time course of the ImGluR(V) amplitude for the experiment shown in A. D, Summary data of ImGluR(V) amplitude normalized to that recorded at 5 min DHPG in seven cells. E, Shift of holding currents in eight cells recorded with Cs+-filled pipettes and voltage clamped at −50 mV. Time 0 is the onset of successful whole-cell patch recording. Plots were well fitted with single exponentials (R2 > 0.91).