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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Dev Biol. 2007 Aug 17;311(1):124–135. doi: 10.1016/j.ydbio.2007.08.023

Figure 6.

Figure 6

Inhibition of Wnt/β-catenin signaling in the AER of the hindlimbs of Rspo2(-/-) mouse embryos. (A-C) LacZ expression in the AER of the left hindlimbs of TopGAL transgenic mouse embryos at E10.5 (A and B) and E11.5 (C). The left hindlimds (HL) are presented in B and C. (D-L) RNA expression of Axin2, a β-catenin target gene, in the AER of the left hindlimbs of Rspo2(+/+), Rspo2(+/-), and Rspo2(-/-) mouse embryos at E11.5. The ventral side (D-F) and leftside (G-I) views of the hindlimbs are presented. The red dotted lines indicate the sectioning planes for the sections presented in J-L. (J-L) Transverse sections of the left hindlimbs of mouse Rspo2 mutant embryos stained with Axin2 riboprobe. Red brackets and arrowheads indicate Axin2 expression in mesenchymal cells and the AER, respectively. (M-Q) RNA expression of Sp8, another β-catenin downstream gene, in the left hindlimbs of Rspo2(+/+), Rspo2(+/-), and Rspo2(-/-) embryos at E10.5 (M-O) and E11.5 (P-Q). (R-T) Wnt3 RNA expression in the left hindlimbs of Rspo2(+/+), Rspo2(+/-), and Rspo2(-/-) embryos at E11.5. Red arrowheads indicate the expression of markers in the AER.