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. Author manuscript; available in PMC: 2009 Jul 1.
Published in final edited form as: Antiviral Res. 2008 Mar 17;79(1):19–27. doi: 10.1016/j.antiviral.2008.02.003

Figure 4. Effect of ETAR on intracellular GTP levels in Vero E6 cells.

Figure 4

Vero E6 cells were treated with either 42 μM [3H]ETAR for 0, 4, 24, 48, and 72 hours (Panel A) or 8, 21, 42, 84, 210, or 420 μM [3H]ETAR for 24 hours (Panel B). Acid-soluble extracts of cell pellets were analyzed by strong anion exchange (SAX) HPLC to determine intracellular GTP and ATP concentrations. The intracellular GTP level for untreated cells was 204 pmole GTP per nmole ATP. Each number represents the mean ± standard deviation from 3 measurements.