Figure 3. VRP-DC vaccines stimulate robust serum levels of neu-specific IgG.

FVB/N mice (n = 6 mice per group) were vaccinated with 10⁶ null- or neuET-DCs, and similarly boosted two weeks later. At seven days post-boost, sera was harvested and used to stain 3T3 cells or 3T3neu cells, followed by staining with goat anti-mouse IgG-FITC. A. Representative staining of 3T3 cells (shaded histogram) or 3T3neu cells (heavy line) with sera from mice vaccinated with either null-DCs or neuET-DCs. B. Quantification of serum anti-neu IgG in vaccinated mice. Bars represent the mean +/− SEM. *p<0.001, Student’s t test.