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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Mol Immunol. 2008 Jul 9;45(14):3731–3739. doi: 10.1016/j.molimm.2008.06.003

Fig. 4.

Fig. 4

Effect of APE/Ref-1 AS oligonucleotide on cell cycle phases of B cells activated by CD40 cross-linking. B cells transfected with APE/Ref-1 AS or control were intracellularly stained with PI and analyzed by flow cytometry to determine the cell cycle status. (A) Representative DNA histograms of B cells transfected with control (APE/Ref-1 ASREV) or APE/Ref-1 AS oligonucleotide were untreated or pre-treated with NAC and then stimulated for 24 h with anti-CD40 mAb. The percentage of cells in each phase of the cell cycle±S.D. from three independent experiments is indicated. (B) Histograms showing the means±S.D. of percentage of cells at G0/G1, S and G2/M of B cell transfected with control or APE/Ref-1 AS oligonucleotides untreated or pre-treated with NAC for 24 h from three independent experiments.