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. Author manuscript; available in PMC: 2009 Dec 4.
Published in final edited form as: Oncogene. 2009 Jun 4;28(22):2219–2230. doi: 10.1038/onc.2009.96

Figure 1. MyoGEF is required for the invasion activity of MDA-MB-231 cells.

Figure 1

(A) Immunoblot analysis with anti-MyoGEF antibody shows that MyoGEF is expressed in MDA-MB-231 and MDA-MB-435S cells, but not in MDA-MB-361, MDA-MB-468, and MCF-7 cells. (B) Immunoblot analysis confirms the depletion of MyoGEF in MDA-MB-231 cells by RNAi. (C) MDA-MB-231 cells depleted of MyoGEF and/or NMIIA were subjected to Matrigel invasion assays. (D) Images in (C) were quantitated by using the NIH ImageJ program. (E) Immunohistochemical analysis of a breast cancer tissue array with MyoGEF antibody. Three arrays were analyzed independently and similar results were obtained. Immunohistochemistry with preimmune serum shows light, background straining (data not shown). Images in (C) and (E) were taken by using a 20x objective (Leica DMI 6000 B microscope).