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. Author manuscript; available in PMC: 2009 Dec 4.
Published in final edited form as: Oncogene. 2009 Jun 4;28(22):2219–2230. doi: 10.1038/onc.2009.96

Figure 2. Depletion of MyoGEF represses RhoA and RhoC activation in MDA-MB-231 cells.

Figure 2

(A) Immunoblot analysis confirms the depletion of MyoGEF in MDA-MB-231 cells by RNAi. (B) The image in (A) was quantitated by using the NIH ImageJ program to estimate the efficiency of MyoGEF depletion in MDA-MB-231 cells by RNAi. (C-F) Depletion of MyoGEF decreases the amount of active RhoA (C) and RhoC (D), but not Rac1 (E) and CDc42 (F), in MDA-MB-231 cells. ~6% of transfected cell lysates were used as control to estimate the amount of total RhoA, RhoC, Rac1, and Cdc42. (G) The images in (C), (D), (E), and (F) were quantitated by using the NIH ImageJ program.