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. Author manuscript; available in PMC: 2009 Dec 4.
Published in final edited form as: Oncogene. 2009 Jun 4;28(22):2219–2230. doi: 10.1038/onc.2009.96

Figure 3. In vitro activation of RhoA, RhoC, and Rac1, but not Cdc42, by MyoGEF.

Figure 3

(A-D) The immunoprecipitated Myc-MyoGEF from transfected HeLa cells could activate RhoA (A), RhoC( B), and Rac1 (C), but not Cdc42 (D) in a fluorescence-based GEF assay. (E) ThioHis-MyoGEF (full-length) could bind both GDP-RhoA (lane 4) and GTP-RhoA (lane 5). (F) A MyoGEF fragment (amino acids 71-388) that contain the DH domain could bind both GDP-RhoC (lane 4) and GTP-RhoC (lane 5). (G) ThioHis-MyoGEF could bind GTP-Rac1 (lane 5) but not GDP-Rac1 (lane 4). D, preloaded with GDP; T, preloaded with GTP.