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. Author manuscript; available in PMC: 2009 Dec 4.
Published in final edited form as: Oncogene. 2009 Jun 4;28(22):2219–2230. doi: 10.1038/onc.2009.96

Figure 5. MyoGEF interacts with NMIIA.

Figure 5

(A) MDA-MB-231 cells expressing Myc-MyoGEF were subjected to immunoprecipitation with anti-Myc antibody followed by immunoblot analysis with anti-IIA or anti-IIB antibodies. Note that Myc-MyoGEF binds to NMIIA but not NMIIB. (B) Schematic diagram of MyoGEF fragments that were used in (C). (C) Interactions between Myc-tagged MyoGEF fragments and endogenous NMIIA. Full-length MyoGEF (lane 3) as well as MyoGEF fragments Myc-PH (lane 5), Myc-1-409 (lane 8), and Myc-1-500 (lane 9) could pull down a significant amount of endogenous NMIIA. Note that cell lysate from lane 3 was also used for immunoprecipitation with normal IgG (lane 2). ~5% of cell lysates were loaded.