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. Author manuscript; available in PMC: 2009 Dec 4.
Published in final edited form as: Oncogene. 2009 Jun 4;28(22):2219–2230. doi: 10.1038/onc.2009.96

Figure 6. Depletion of MyoGEF by RNAi impairs MDA-MB-231 cell polarity.

Figure 6

(A) MDA-MB-231 cells treated with control siRNA (siCont) or MyoGEF siRNA (siMyoGEF) for 48 h were trypsinized, replated on fibronectin-coated coverslips, and cultured for an additional 6 h. Note that cells depleted of MyoGEF did not polarize. (B) Quantitation of nonpolarized MDA-MB-231 cells treated with control or MyoGEF siRNAs. (C) MDA-MB-231 cells treated with siCont or siMyoGEF were subjected to immunofluorescence with MyoGEF antibody (red) and FITC-phalloidin (green). (D) MDA-MB-231 cells treated with siCont or siMyoGEF were stained with antibodies specific for p-MRLC (green) and NMIIA (red). (E) MDA-MB-231 cells treated with siCont or siMyoGEF were stained with antibodies specific for p-MRLC (green) and NMIIB (red). Bar in (A), 80 μm; Bars in (C), (D), and (E), 10 μm