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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: J Neurochem. 2009 Feb 5;109(1):257–267. doi: 10.1111/j.1471-4159.2009.05957.x

Fig. 1.

Fig. 1

Preconditioning (PC) of cultured rat cortical neurons made these cells more resistant to a harmful OGD. Cortical neurons were subjected to a brief OGD (30 min or 60 min) as preconditioning and 24 h later to a harmful OGD (4 h). After 16 h ROG, cell death was assessed. (a) Representative fields of cortical neuronal culture subjected to the various conditions indicated and stained with Hoechst 33342 and PI. In the center inset, nuclear stains show examples of live cells designated by arrowheads and dead cells shown by thin arrows. (b) Quantitative data of cell death (total apoptotic and necrotic) shown as percentage of total cell population in different experimental conditions (left panel) and by LDH release as percentage of total LDH (right panel). Data are shown as means ± SD (n = 4). **p < 0.01, *p < 0.05 compared with cells subjected to a harmful OGD without PC.