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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: J Neurochem. 2009 Feb 5;109(1):257–267. doi: 10.1111/j.1471-4159.2009.05957.x

Fig. 5.

Fig. 5

The depletion of SUMO-1 made SHSY5Y cells more sensitive to OGD/ROG and the cells that were depleted of SUMO-1 were not protected by preconditioning. (a) SUMO-1 (left panel) and SUMO-2/3 (right panel) protein levels in total cell lysates from SHSY5Y cells transfected with various shRNA constructs as follows: 1, empty vector; 2, SUMO-1 sh#2; 3, SUMO-2 sh#1; 4, SUMO-2 sh#1 plus SUMO-3 sh#1; 5, SUMO-2 sh#1 plus SUMO-3 sh#2; 6, SUMO-2 sh#1 plus SUMO-3 sh#3. Upper panel: a representative PAGE/WB picture, lower panels: quantitative analyses; solid bars:conjugates; striped bar: free forms. Data are shown as means ± SD (n = 6). **p < 0.01, *p < 0.05 compared with endogenous levels. (b) Sensitivities to OGD/ROG among shRNA transfected SHSY5Y cells. Data here are shown as means ± SD (n = 6). **p < 0.01 compared with the cells transfected with empty vector. (c) SHSY5Y cells transfected with empty vector or SUMO-1 sh#2 were subjected to a harmful OGD/ROG with or without preconditioning (4 h OGD), and assayed for cell death. Cell death in control and PC alone are also included. Data are shown as means ± SD (n = 3).