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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: J Neurochem. 2009 Feb 5;109(1):257–267. doi: 10.1111/j.1471-4159.2009.05957.x

Fig. 7.

Fig. 7

The down-regulation of SUMO-1 made mouse cortical neurons more sensitive to OGD/ROG and the SUMO-1-depleted neurons show reduced protection by preconditioning. (a) SUMO-1 (left panel) and SUMO-2/3 (right panel) protein levels in total cell lysates from mouse cortical neurons treated with various siRNAs. Upper panels: representative PAGE/WB pictures. Lower panels: quantitative analyses of densities. Data are shown as means ± SD (n = 3). (b) Sensitivities to OGD/ROG among siRNA-treated cells. Cell death was measured with Hoechst 33342 and PI followed by FACS analysis. Data here are shown as means ± SD (n = 3). (c) Mouse cortical neurons that had been transfected with AllStar siRNA or SUMO-1 siRNA were subjected to a harmful OGD/ROG with or without preconditioning (30 min OGD). Cell deaths were measured as mentioned above. Data here are shown as means ± SD (n = 3).