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Effect of palmitic acid on myotube cytokine mRNA expression. Differentiated myotubes were treated with 0.2 mM palmitic acid conjugated to bovine serum albumin (BSA) (M+P) or with BSA control (M) for 24 h. mRNA levels were quantified by real-time RT-PCR and were normalized to endogenous 18S RNA. Data (n = 8) are means ± SE. *P ≤ 0.05 vs. M. TNF-α, tumor necrosis factor-α; IL, interleukin; IL-1RA, IL-1 receptor antagonist.
