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. 2009 Mar 31;296(6):E1300–E1310. doi: 10.1152/ajpendo.90885.2008

Fig. 5.

Fig. 5.

Effect of macrophages and palmitic acid on potential mediators of inflammation. A: effect of palmitic acid on toll-like receptor TLR-4 and TLR-2 mRNA expression. Differentiated myotubes were cocultured with macrophages (MMc) or, as a control for the coculture, with fibroblasts (MF) for 48 h. Cocultures were treated without or with (+P) 0.2 mM palmitic acid for 24 h before collection of cell lysates. Data are means ± SE. *P < 0.01 vs. MMc, †P < 0.05 vs. MF. B and C: effect of macrophages and palmitic acid on IκB-α (B) and phospho (p)JNK1 and JNK (C) protein expression in myotube cocultures. Representative Western blots of myotube cell lysates after coculture with fibroblasts (F, MF) or macrophages (Mc, MMc) in the absence or presence (+P) of 0.2 mM palmitic acid for 48 h are shown. Bar plots are means ± SE of densitometric analysis of the Western blots normalized to β-actin from myotubes from 6 different subjects. *P ≤ 0.05 MMc+P vs. MMc, **P < 0.03 MMc vs. MF, †P < 0.01 MF+P vs. MF.