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XZ-images of 100-nm-diameter fluorescent beads (a and b) and of 100-nm-diameter negatively stained glass beads agglomerations (c and d) as observed in the confocal (a and c) and the STED-fluorescence (b and d) microscope. Note the artifacts indicated by arrows induced by the elongated spot in the confocal image and their reduction in the STED counterpart.
