Fig. 3.

Intracellular Cys are necessary for the nitric oxide effect. A–D: current-voltage (I-V) plots of normalized currents recorded in oocytes expressing wild-type Cx46 and Cx46 mutants under control conditions (•) or after exposure to 1 mM GSNO (○). Currents were measured at the end of the 15-s pulses and were normalized to the +60-mV value. The Boltzmann equation (see text) was fitted to the data. Continuous line, control; segmented line, GSNO. See Fig. 2 for additional details.