Fig. 7.

GSNO affects dye uptake through Cx46 but not Cx46-C3A hemichannels. Oocytes expressing wild-type Cx46 or Cx46-C3A were exposed to 2 mM 5(6)-carboxyfluorescein (CF), 1 mM Lucifer yellow (LY), or 1 mM ethidium bromide (EthBr), indicated at the top, for 40 min, in the absence or presence of 1 mM GSNO. After treatment, the oocytes were washed and sonicated and the dye content was measured by fluorometry. Data depicted are the normalized effects of 1 mM GSNO on dye uptake by oocytes in ND96 (1.8 mM Ca²⁺, open bars) or in the nominal absence of divalent cations (hatched bars). For the normalization, the dye uptake in GSNO was divided by that in the absence of GSNO at normal or low [Ca²⁺]. Data are from 3 independent experiments for each dye (8 oocytes per experiment). *P < 0.05, control condition vs. 1 mM GSNO.