Fig. 4.

Voltage dependent activation and inactivation of the anti-K_v2.1-sensitive current. A: voltage-dependent inactivation of the anti-K_v2.1-sensitive current of K_v2.1 or K_v2.1/K_v9.3 heterologous expressed. The inactivation curve was obtained as in Fig. 3H. For comparison, the inactivation curves of K_v2.1 and K_v2.1/K_v9.3 obtained from heterologous expression are also shown. B: outward K⁺ current traces of K_v2.1/K_v9.3 expressed in Ltk⁻ cells before (solid line) and after (dotted lines) diffusion of K_v2.1 antibodies into the cell. The voltage protocol is shown in the inset. C: representative current tracings of the outward K⁺ current of DRG neurons before (solid line) and after (dotted lines) intracellular diffusion of K_v2.1 antibodies. The same voltage protocol as in B was used. D: inactivation curve of the anti-K_v2.1-sensitive current was obtained as in Fig. 3H. Data were fitted with a double Boltzmann function (solid line). The inactivation curves of wild-type K_v2.1 and wild-type K_v2.1/K_v9.3 expressed in Ltk⁻ cells are also shown. The midpoints of inactivation and slope factors are shown in Table 1.