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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Nitric Oxide. 2008 Feb 15;18(4):256–265. doi: 10.1016/j.niox.2008.02.002

Figure 8.

Figure 8

CO enhances phosphorylation of p38 MAPK, while suppressing Akt phosphorylation in IL-1β-treated hepatocytes. (A) Cells were starved for 24h in medium without serum and stimulated with IL-1β (500 U/ml) in the presence or absence of CO (250 ppm). Cell lysed in lysis buffer containing with inhibitor of phosphatase and protease. Western blot was performed from cytosolic fraction using denaturing SDS-PAGE. The SB203580 reversed the inhibitory effect of CO on IL-1β-induced iNOS expression and nitrite production. (B) Cells were incubated with inhibitors of p38 or ERK (SB;10 μM, PD; 25 μM) for 1h and further incubated with IL-1β for 24h in a presence or absence of CO (250 ppm).