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. 2009 Mar 18;296(6):F1417–F1427. doi: 10.1152/ajprenal.90337.2008

Fig. 3.

Fig. 3.

Chronic treatment with TGF-α (20 ng/ml) or EGF (100 ng/ml) reduces α ENaC subunit protein in the apical membrane in 2F3 cells. A: representative immunoblot of α-ENaC protein in the apical membrane and in whole cell lysates. B: summary graph showing the change in band densities of α-ENaC in the apical membrane after treatment with TGF-α and EGF. Dashed line represents control level, which is set at 100%. C: summary graph showing the change in band densities of α-ENaC on the whole cell level after treatment with TGF-α and EGF. Dashed line represents control level, which is set at 100%. *P < 0.05 vs. Con.