Fig. 4.

Chronic treatment with TGF-α (20 ng/ml) or EGF (100 ng/ml) does not change the level of β-ENaC in the apical membrane in 2F3 cells. A: representative immunoblot of β-ENaC protein in the apical membrane and in whole cell lysates. B: summary graph showing the change in band densities of β-ENaC in the apical membrane after treatment with TGF-α and EGF. Dashed line represents control level, which is set at 100%. C: summary graph showing the change in band densities of β-ENaC in whole cell lysates after treatment with TGF-α and EGF. Dashed line represents control level, which is set at 100%. *P < 0.05 vs. Con.