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NFAT5 contributes to TNF production in mTAL cells. mTAL cells were incubated in the absence or presence of 1.2 mM CaCl2 for 9 h following transfection with pcDNA3.1-NFAT5 or the empty plasmid vector without the NFAT5 cDNA (pcDNA3.1). Supernatants were harvested, and TNF concentrations were determined by ELISA. Values are means ± SD. P < 0.05; n = 3.
