Fig. 5.
Inhibition of NFAT5 blocks TNF promoter activity in mTAL cells. mTAL cells (5 × 105) were incubated in the absence or presence of 1.2 mM CaCl2 for 9 h after transfection with a TNF-luciferase promoter construct and either a small hairpin (sh)RNA vector targeting NFAT5 at exon 8 (U6-N5 ex8) or dominant negative calcium-sensing receptor (CaR; CaR-DN; A) or dominant negative NFAT5 (NFAT5-DN; B). Cells were harvested after stimulation, and firefly luciferase and Renilla activities were assayed; corresponding empty or scrambled vectors were included as controls. Values are means ± SD. P < 0.05; n = 3.