Table 1.
Oligonucleotide primers for polymerase chain reaction of murine isoforms of NFAT
| Primer | Sequence | PCR Size, bp | Position | Reference |
|---|---|---|---|---|
| F1-NFAT1m | 5-CTACATGGAGAACAAGCCT-3 | 1439–1457 | (mice)(cDNA) NM_010899 | |
| R1-NFAT1m | 5-GTTTCGGAGCTTCAGGATGC-3 | 228 | 1667–1648 | (mice)(cDNA) NM_010899 |
| F1-NFAT2m | 5-CCTTCGGAAGGGTGCCTTTT-3 | 1317–1297 | (mice)(cDNA) NM_016791 | |
| R1-NFAT2m | 5-CGGCTGCCTTCCGTCTCATAG-3 | 215 | 1638–1618 | (mice)(cDNA) NM_016791 |
| F1-NFAT3m | 5-CATTGGCACTGCAGATGAG-3 | 1430–1448 | (mice)(cDNA) NM_023699 | |
| R1-NFAT3m | 5-CGTAGCTCAATGTCTGAAT-3 | 209 | 1639–1621 | (mice)(cDNA) NM_023699 |
| F1-NFAT4m | 5-CTACTGGTGGCCATCCTGTTGT-3 | 1383–1404 | (mice)(cDNA) NM_010901 | |
| R1-NFAT4m | 5-ACTTTTGTGCTGGCGATTAT-3 | 183 | 1566–1547 | (mice)(cDNA) NM_010901 |
| F1-NFAT5m | 5-AACATTGGACAGCCAAAAGG-3 | 804–823 | (mice)(cDNA) NM_018823 | |
| R1-NFAT5m | 5-GCAACACCACTGGTTCATTA-3 | 223 | 1027–1008 | (mice)(cDNA) NM_018823 |
NFAT, nuclear factor of activated T cells.