Fig. 9.

Reduced P2X7 protein and a reduced index of P2X7 function in ADPKD cyst cells. P2X7 immunostaining in DBA⁺-NK cells (A), DBA⁺-PKD cells (B), and DBA⁺-NK cells in the added presence of peptide antigen (C). Scale bar = 10 μm. D: biphasic effect of 1 mM extracellular ATP on [Ca²⁺]_i in 500 nM thapsigargin-treated NK_TERT cells (○) and PKD_TERT cells (•) and in thapsigargin-treated NK_TERT cells pretreated with and in the continued presence of the P2X7 antagonist oxidized ATP (O-ATP; 300 μM, ▵).