Fig. 1.

Genomic evaluation of mice used in this study. A: location of loxP sites relative to exons 2–11 of the Rh C glycoprotein (Rhcg) gene. B: representative examination of loxP sites. Primers flanking the 3′-loxP site are used to amplify genomic DNA. Amplification of an allele without an inserted loxP site results in a product of ∼230 bp, whereas when a loxP site is present a ∼370-bp product is amplified. C: PCR amplification of genomic DNA using primers flanking both loxP sites. In the absence of genomic recombination, an ∼3.6-kb product is amplified. In the presence of recombination, genomic DNA between the loxP sites is removed and amplification using primers flanking the loxP sites results in a product of ∼750 bp. Amplification of DNA from cortex and outer medulla shows no recombination in Cre-negative mice and recombination in Cre-positive mice. No recombination is present in tail DNA from either Cre-negative or Cre-positive mice.