Fig. 3.

Serial sectioning of Rhcg immunolabel with TSC/AQP2 label. A: Rhcg immunolabel in the cortex of collecting duct-specific Rhcg deletion (CD-KO) mouse on a normal diet. B: serial section double-labeled for AQP2 (brown) and TSC (blue). Residual Rhcg immunolabel is present in a subpopulation of cells in both CNT and DCT. C and D: similar findings [Rhcg immunolabel (C), AQP2 and TSC double-immunolabel (D)] in the cortex of acid-loaded CD-KO mice. Again, residual Rhcg immunolabel is present in a subpopulation of cells in both CNT and DCT. No Rhcg immunolabel is present in cortical collecting duct (CCD) from mouse on either a normal diet or after acid loading.