Fig. 4.

GT1–7 cells exhibit daily changes in sensitivity to kisspeptin and VIP stimulation. Post-transcriptional regulation of GnRH in GT1–7 cells is responsive to kisspeptin or VIP treatment at various time points up to 25 h after serum shock. Data are presented as the fold increase above untreated samples for each time point. Error bars represent standard errors (n = 4). ∗ Significantly greater than the lowest value over the course of the sampling period: p < 0.05. Kisspeptin and VIP treatments showed a similar stimulation pattern across the sampling period, with cellular GnRH peptide showing changes in expression level to the stimulation over the course of the sampling period.