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. Author manuscript; available in PMC: 2009 Dec 4.
Published in final edited form as: Oncogene. 2009 Apr 27;28(22):2196–2204. doi: 10.1038/onc.2009.100

Figure 5. Stabilization of p53 by Artemis depletion is induced by oxidative stress derived from mitochondrial respiration.

Figure 5

(A) Immunoblots showing that reduction of oxygen tension from 21% to 3% abrogates stabilization of p53 by Artemis depletion in primary cell lines. (B) The antioxidant NAC (10 mM) inhibits p53 stabilization induced by Artemis depletion. (C) Rotenone (0.04 mM) and TTFA (0.2 mM), inhibitors of mitochondrial electron transport, reduce the stabilization of p53 induced by Artemis depletion. (D,E) p53 responsive oxidative stress genes, TIGAR and Sestrin 2, are upregulated by Artemis depletion, and this effect is suppressed by co-depletion of DNA-PKcs.