Figure 3. Mechanism of nuclear c-Rel enhancement is not dependent on disruption of cytoplasmic IκB:c-Rel interactions.

A) ΔNp63 overexpression does not alter total levels of cellular NF-κB. Western blots of whole cell lysates from primary mouse keratinocytes harvested 21h post-adenoviral introduction of human ΔNp63α or β-gal. Cultures were maintained in medium containing 0.05mM Ca²⁺ or exposed to 0.12 mM Ca²⁺ for the final 4h. B) Levels of IκB regulatory proteins are not decreased in ΔNp63α-overexpressing keratinocytes that accumulate nuclear c-Rel. Western blot of whole cell lysates of keratinocytes overexpressing β-gal or ΔNp63α. C) ΔNp63α overexpression does not inhibit normal cytoplasmic interactions between c-Rel and the IκB proteins. Co-immunoprecipitation analysis of whole cell lysates from keratinocytes overexpressing ΔNp63α or β-gal.