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. Author manuscript; available in PMC: 2009 Jun 8.
Published in final edited form as: Eur J Neurosci. 2009 Mar;29(6):1119–1130. doi: 10.1111/j.1460-9568.2009.06664.x

FIG. 1.

FIG. 1

Murine recombinant 3K3A-APC blocks NMDA-induced apoptosis in mouse cortical neurons. (a) Immunostaining for terminal deoxynucleotidyl transferase-mediated digoxigenin-dUTP nick-end labeling (TUNEL) and Hoechst at 24:00 h after NMDA in the absence or presence of murine recombinant 3K3A-APC (5 nm). (b) Dose-dependent neuroprotective effects of murine 3K3A-APC (green) and wt-APC (yellow) at 24:00 h of NMDA (*P < 0.01 by two-way ANOVA). Cell survival was quantified with a WST-8 assay. NMDA label at zero concentration APC on the abscissa shows cell survival of neurons treated with NMDA only without 3K3A-APC or wt-APC. Vehicle denotes the basal cell survival rate of neurons in the culture medium in the absence of NMDA. S360A-APC indicates cell survival of neurons treated with NMDA in the presence of enzymatically inactive APC (negative control, brown). (c) IC50 (inhibiting concentration) values for 3K3A-APC vs. wt-APC were calculated from experiments shown in (b). All values are mean ± SEM.