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. Author manuscript; available in PMC: 2009 Oct 2.
Published in final edited form as: Nature. 2009 Feb 22;458(7238):591–596. doi: 10.1038/nature07849

Figure 2.

Figure 2

Eya interacts with H2AX in a DNA-damage dependent manner. (a) HA-tagged Eya1 or Eya3 interacts with FLAG-tagged H2AX in 293T cells in response to IR (5Gy), but not under basal conditions. (b) Co-immunoprecipitation experiments examining endogenous Eya3 protein using a specific Eya3 antibody recapitulated that interaction data for the tagged proteins. (c) Using sonicated chromatin as input, co-immunoprecipitation experiments showed that HA-Eya1/3 interacts with H2AX on chromatin. Immunostaining of 293T cells demonstrates that transfected, HA-tagged Eya1 (d) or Eya3 (e) localizes to DNA-damage induced foci coincident with γH2AX specifically after treatment with IR (5Gy, 1hr). Representative examples of foci formation are shown.